HA/TCP 골이식재상에 이식된 지방유래 줄기세포의 골모세포로의 분화 및 골형성에 대한 연구
Bine Regeneration with Adipose Tissue-Derived Mesenchymal Stem Cell and HA/TCP
임재석, 이의석, 장현석, 이태형, 정유민, 박정균, 권종진,
소속 상세정보
임재석 ( Rim Jae-Suk ) - 고려대학교 의과대학 구강악안면외과학교실
이의석 ( Lee Eui-Seok ) - 고려대학교 의과대학 구강악안면외과학교실
장현석 ( Jang Hyon-Seok ) - 고려대학교 의과대학 구강악안면외과학교실
이태형 ( Lee Tai-Hyung ) - 고려대학교 의과대학 구강악안면외과학교실
정유민 ( Jeong You-Min ) - 고려대학교 의과대학 구강악안면외과학교실
박정균 ( Park Jeong-Kyun ) - 고려대학교 의과대학 구강악안면외과학교실
권종진 ( Kwon Jong-Jin ) - 고려대학교 의과대학 구강악안면외과학교실
KMID : 0360120100320020097
Abstract
Aim of the study: An alternative source of adult stem cells that could be obtained in large quantities, under local anesthesia, with minimal discomfort would be advantageous. Adipose tissue could be processed to obtain a fibroblast-like population of cells or adipose tissue-derived stromal cells (ATSCs). This study was performed to confirm the availability of ATSCs in bone tissue engineering.
Materials and Methods: In this study, adipose tissue-derived mesenchymal stem cell was extracted from the liposuctioned abdominal fat of 24-old human and cultivated, and the stem cell surface markers of CD 105 and SCF-R were confirmed by immunofluorescent staining. The proliferation of bone marrow mesenchymal stem cell and ATSCs were compared, and evaluated the osteogenic differentiation of ATSCs in a specific osteogenic induction medium. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific BMP-2, ALP, Cbfa-1, Osteopontin and osteocalcin were confirmed by RT-PCR. With differentiation of ATSCs, calcium concentration was assayed, and osteocalcin was evaluated by ELISA (Enzyme-linked immunosorbant assay). The bone formation by 5-week implantation of HA/TCP block loaded with bone marrow mesenchymal stem cells and ATSCs in the subcutaneous pocket of nude mouse was evaluated by histologic analysis.
Results: ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. ATSCs could be easily identified through fluorescence microscopy, and bone formation in vivo was confirmed by using ATSC-loaded HA/TCP scaffold.
Conclusions: The present results show that ATSCs have an ability to differentiate into osteoblasts and formed bone in vitro and in vivo. So ATSCs may be an ideal source for further experiments on stem cell biology and bone tissue engineering.
키워드
Adipose tissue-derived stromal cells;ATSCs;Bone tissue engineering;Osteogenic medium
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